Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Central to the better understanding of both molecular mechanisms and disease, cell migration plays an essential role in a variety of biological processes and is now the subject of intense study using an array of powerful new technologies. In Cell Migration: Developmental Methods and Protocols, researchers describe in step-by-step detail their most successful techniques for studying the macromolecular machinery of cell movement. These readily reproducible protocols include a wide range of novel and state-of-the-art methodologies, as well as many classic methods, for use in cultured cells, different model organisms, and specialized cells in both normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The authors also offer several novel approaches to the study of cell migration, as well as extensive coverage of cell migration studies in developmental and disease models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Cell Migration: Developmental Methods and Protocols offers researchers easy access to many readily reproducible techniques for the optimally productive investigation of cell migration in today's interdisciplinary experimental environment.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

International Review of Cytology presents current advances and comprehensive reviews in cell biology - both plant and animal. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. Articles in this volume address the pathophysiological dynamics of human ovarian surface epithelial cells in epithelial ovarian carcinogenesis; morphodynamics of the secretory pathway; aspects of collagen mineralization in hard tissue frmation; the hypoxia-inducible factor and tumor progression along the angiogenic pathway; multidrug

Biology --- Cytology. --- Biology. --- Life sciences --- Biomass --- Life (Biology) --- Natural history --- Cell biology --- Cellular biology --- Cells

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Powerful new techniques in molecular biology have revolutionized our ability to investigate and define cellular processes at the molecular level for medical purposes. In Medical Biomethods Handbook, John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are Southern and Western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy. State-of-the-art and highly practical, Medical Biomethods Handbook offers novice and experienced researchers an in-depth understanding of the theory, practice, and application of the key molecular biology techniques used in biomedical research and diagnostic investigation today.

Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Biosciences --- Sciences, Life --- Science

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Use of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies.

Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Nuclear Import and Export in Plants and Animals provides insight into the remarkable mechanisms of nuclear import and export. This book covers a range of topics from the nuclear pore structure, to nuclear import and export of macromolecules in plant and animal cells. In addition, the book covers the special cases of nuclear import of Agrobacterium T-DNA during plant genetic transformation, nuclear import and export of animal viruses, and nuclear intake of foreign DNA. A chapter on research methods to study nuclear transport concludes the book.

Nuclear membranes. --- Biological transport. --- Proteins --- Physiological transport. --- Membrane transport --- Passive transport, Biological --- Physiological transport --- Transport, Biological --- Diffusion --- Osmosis --- Nuclear envelope --- Cell membranes --- Cell nuclei --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cell biology.